Saos-2-CAS9 cell line

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  • Saos-2-CAS9 cell line Saos-2-CAS9-产品图
Saos-2-CAS9 cell line Saos-2-CAS9-产品图
Saos-2-CAS9 Copy Product Info
Catalog#： YC-D017-Cas9-H
Size： 1*10^6
Culture method： 85%McCOY's 5A+15% FBS
STR Report
  • Saos-2-CAS9 cell line Saos-2-CAS9-产品图
Saos-2-CAS9 Copy Product Info
Catalog#： YC-D017-Cas9-H
Size： 1*10^6
Culture method： 85%McCOY's 5A+15% FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 85%McCOY's 5A+15% FBS
Passage ratio 1:2~1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
85%McCOY's 5A+15% FBS
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's Saos-2 cell line was constructed on the basis of wild-type Saos-2 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in Saos-2 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

Cutting Efficiency Validation
Saos-2-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the Saos-2-CAS9 stable cell pool which is electroporated by SDHB gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:Saos-2		 STR Info (Cell bank)
Cell Line:SAOS-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 12131213
D7S820 810810
D16S539 12131213
VWA 18181818
TH01 6969
AMEL XXXX
TPOX 8888
CSF1PO 10101010
D12S391 2020
FGA 2225
D2S1338 1818
D21S11 282930.0
D18S51 1515
D8S1179 1012
D3S1358 1418
D6S1043 1114
PENTAE 1419
D19S433 1313
PENTAD 1112
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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